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Title
 Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
Article ID Volume Year Page Start Issue Type
  5880   124   2000   1139   3   research_article
PubSource TAIR Reference ID
  # 2988 PLANT PHYSIOLOGY  5882
Scanned Date Scanned By Is Local Hard Is Downloaded Is Scanned
  2021-10-26   UniProt   n   y   y
Ref Text Is Peer Reviewed IsPrintRef IsE-Ref
    y   y
Link Doi
  https://pubsearch.arabidopsis.org/pdfs/5880.pdf   http://doi.org/10.1104/pp.124.3.1139
Authors
  Fujiki, Y.,Ito, M.,Nishida, I.,Watanabe, A.
Abstract
  We have identified many dark-inducible (din) genes that are expressed in Arabidopsis leaves kept in the dark. In the present study we addressed the question of how plant cells sense the depletion of sugars, and how sugar starvation triggers din gene expression in suspension-cultured cells of Arabidopsis. Depletion of sucrose in the medium triggered marked accumulation of din transcripts. Suppression of din gene expression by 2-deoxy-Glc, and a non-suppressive effect exerted by 3-O-methyl-Glc, suggested that sugar-repressible expression of din genes is mediated through the phosphorylation of hexose by hexokinase, as exemplified in the repression of photosynthetic genes by sugars. We have further shown that the signaling triggered by sugar starvation involves protein phosphorylation and dephosphorylation events, and have provided the first evidence that multiple pathways of protein dephosphorylation exist in sugar starvation-induced gene expression. An inhibitor of serine/threonine protein kinase, K-252a, inhibited din gene expression in sugar-depleted cells. Okadaic acid, which may preferentially inhibit type 2A protein phosphatases over type 1, enhanced the transcript levels of all din genes, except din6 and din10, under sugar starvation. Conversely, a more potent inhibitor of type 1 and 2A protein phosphatases, calyculin A, increased transcripts from din2 and din9, but decreased those from other din genes, in sugar-depleted cells. On the other hand, calyculin A, but not okadaic acid, completely inhibited the gene expression of chlorophyll a/b-binding protein under sugar starvation. These results indicate that multiple signaling pathways, mediated by different types of protein phosphatases, regulate gene expression during sugar starvation.
Annotations
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8 Annotations
AT3G50210 acts upstream of or within cellular response to starvation (GO:0009267) by TAS : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: linda/2002-07-31
AT3G47340 acts upstream of or within amino acid catabolic process (GO:0009063) by TAS : inferred by author, from enzymatic pathway
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: garcia/2004-05-06
AT3G47340 expressed during sporophyte senescent stage (PO:0007017) by IEP : Transcript levels (e.g. Northerns)
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: garcia/2004-05-06
AT3G49620 acts upstream of or within cellular response to starvation (GO:0009267) by TAS : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: linda/2002-01-30
AT3G49630 acts upstream of or within cellular response to starvation (GO:0009267) by TAS : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: linda/2002-07-31
AT1G67070 involved in response to sucrose (GO:0009744) by IEP : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: UniProt/2012-10-09
AT3G13450 involved in cellular response to sucrose starvation (GO:0043617) by IEP : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: UniProt/2013-04-04
AT3G06850 involved in cellular response to sucrose starvation (GO:0043617) by IEP : none
Evidence from Multiple signaling pathways in gene expression during sugar starvation. Pharmacological analysis of din gene expression in suspension-cultured cells of arabidopsis.
annotated by/date: UniProt/2013-04-04
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MedlineID BiosisID AgricolaID PubMedID PMCentralID PubReferenceID
  20532789     IND22435281   11080291     5882
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