| Pubsearch |
|
[Search for
Articles
/
PubSources
/
Genes
/
Hits
/
Terms
/
Alleles
/
Symbols
/
Community Reference
/
Germplasms
/
Phenotypes
/
All
]
[Find Help Curation Guide / Discussion Mailing List ] |
[Add
Article /
Articles in Bulk /
Gene /
Locus /
Term /
Communication /
Allele /
Symbol /
Community Reference /
Germplasm /
User /
Community Submission
Other Annotations
Bulk Annotation Upload
]
[Browse Gene Ontology / Tair Ontology / Annotation Tasks ] [Submit SourceForge Bug Report ] |
Article Actions: [Update existing article hits / Add new hits]
| Title | ||||||
|---|---|---|---|---|---|---|
| Phenotypic analysis of ASK gene loss of function alleles in Arabidopsis | ||||||
| Article ID | Volume | Year | Page Start | Issue | Type | |
| 45901 | 2008 | abstract | ||||
| PubSource | TAIR Reference ID | |||||
| # 3536 19th International Conference on Arabidopsis Research | 501728110 | |||||
| Scanned Date | Scanned By | Is Local Hard | Is Downloaded | Is Scanned | ||
| Melanie Trull | ||||||
| Ref Text | Is Peer Reviewed | IsPrintRef | IsE-Ref | |||
| n | ||||||
| Link | Doi | |||||
| http://doi.org/ | ||||||
| Authors | ||||||
| *Dezfulian, Mohammad H. Franczk, Marta Goettel, James Dahliwal, Rajdeep Crosby, William L. | ||||||
| Abstract | ||||||
| Genetic studies have indicated the importance of targeted post-translational protein degradation as a key mechanism for the regulation of gene expression in plants. One of the main pathways involved in targeted protein degradation is the Ubiqutin/26S proteasome pathway. One highly studied class of E3 ligase are the SCF class whose quaternary structure typically includes 4 canonical polypeptides - Cullin, Rbx, Skp and an F-box subunit. In Arabidopsis, the Skp family of SCF subunits is encoded by 21 Skp1-like ASK genes compared to 1 orthologous gene in humans or yeast.<br><br><br><br>Phylogenic analyses based on deduced amino acid sequence and expression profiles result in the clustering of the ASK gene family to distinct clades. The phylogeny suggests the possibility of functional redundancy between related Skp proteins. This suggestion is supported by genetic observation that ask1/ask2 double mutant exhibited a marked severe phenotype involving developmental delay and seedling lethality, whereas these defects were not detected in mutant lines carrying either ask1 or ask2 mutants alone<sup>(1)</sup>.<br><br><br><br>To elucidate the function of select members of the ASK gene family in Arabidopsis, we have undertaken a program of functional studies of a subset of the predicted ASK family of genes using two reverse-genetic resources; T-DNA insertions derived from the SIGNaL resource <sup>(2)</sup> targeting selected members of the gene family, and transgenic plants expressing artificial microRNA constructs designed to suppress the abundance of one or more ASK transcripts<sup>(3)</sup>.Example data will be presented describing aberrant plant growth and development phenotypes among select loss-of-function alleles of ASK3 or ASK10 . The genetic resources developed by this study are the basis for further studies into the specific targeting functions and molecular mechanisms by which ASK genes contribute to plant patterning and development.<br><br><br><br><sub><b>1.F. Liu et al., Plant Cell 16, 5 (2004).<br><br><br><br>2.J. M. Alonso et al., Science 301, 653 (2003).<br><br><br><br>3.R. Schwab, S. Ossowski, M. Riester, N. Warthmann, D. Weigel, Plant Cell 18, 1121 (2006).</sub></b> | ||||||
| Annotations | ||||||
Jump to page [ 1 ] |
||||||
| MedlineID | BiosisID | AgricolaID | PubMedID | PMCentralID | PubReferenceID | |
| 1700350 | ||||||
|
Related terms:
|
Related genes:
|
|||||
| comments or questions to: gmod-pubsearch-dv@lists.sourceforge.net. Bug reports can be sent to SourceForge. |