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Search results 2 hits to papers found. Showing 10 , 25 , 50 , 100 items per page Jump to page [ 1 ]
#	Term Name	Article Title and Abstract	Hit details	Hit Valid?
1.	gene AT3G53060.1 ( pub:12000) Alias: F8J2.230 Symbols: ASK6 <SKP1-like 6>  |  SK6 <SKP1-like 6> TAIR:gene:2085200 (Accession)	Article [ 45763 ] (2008) [abstract] Understanding the function of unknown genes in Arabidopsis by quantitative real time PCR expression profiling and promoter-reporter expression analysis in transgenic plants   19th International Conference on Arabidopsis Research *Town, Chris Redman, Julia Zhuang, Jun Monaghan, Erin Wang, Wei Moskal, William Wu, Hank Quan, Hui Underwood, Beverly Xiao, Yongli (TAIR Reference:501727972) A variety of platforms, including cDNA microarray, Affymetrix GeneChip, whole genome tiling array, massively parallel signature sequencing, have been used extensively to study gene expression in Arabidopsis thaliana. However, there are still many genes in the Arabidopsis genome that could not be profiled effectively by any of these methods, generally due to the low abundance of their transcripts in mRNA populations. We are therefore generating expression profiles by quantitative real time PCR (qRT-PCR) which is about 2-3 orders of magnitude more sensitive than hybridization-based technologies for many of the function of unknown genes in Arabidopsis currently lacking reliable expression data. To date, we have performed qRT-PCR on about 2,000 genes using mRNAs from leaf, root, bud, silique and T87 cell culture and seedlings treated with IAA, SA and salt. Over 90% of the genes were expressed in at least one of our current cDNA populations and ~ 40% of them showed differential expression in at least 2 out of 8 conditions. We are using co-expression analysis to associate these low-expressing genes with functionally annotated genes and pathways. In addition, we have developed a high throughput pipeline to generate promoter-reporter constructs and transgenic Arabidopsis plants for a subset of these genes of unknown function. So far, promoters from 587 genes have been cloned into a GFP reporter construct and 495 have been transformed into Arabidopsis. All the GFP expression patterns detected in these transgenic plants are localized to small regions of tissues and cell types. Both the qPCR and reporter construct data can be found at www.tigr.org/tdb/e2k1/ath1/qpcr/index.shtml. Supported by NSF 2010. Scanned By: comm Other associated terms: cell cycle / AT1G75530.1 / AT1G14930.1 / AT1G14750.1 / AT1G33530.1 / AT1G69500.1 / AT1G06970.1 / AT1G03050.1 / AT1G48400.1 / AT1G48670.1 / AT1G48820.1 / AT1G48800.1 / AT1G51220.1 / AT1G51230.1 / AT1G61130.1 / AT1G67770.1 / AT1G35440.1 / AT1G34140.1 / AT1G34540.1 / AT1G34500.1 / AT1G22420.1 / AT1G53370.1 / AT1G53250.1 / AT1G53330.1 / AT1G65990.1 / AT1G49420.1 / AT1G49250.1 / AT1G49270.1 / AT1G12870.1 / AT1G28000.1 / AT1G28040.1 / AT1G56410.1 / AT1G64930.1 / AT1G64820.1 / AT1G54980.1 / AT1G54930.1 / AT1G35730.1 / AT1G52790.1 / AT1G52800.1 / AT1G56030.1 / AT1G49610.1 / AT1G09400.1 / AT1G09410.1 / AT1G09470.1 / AT1G14500.1 / AT1G33670.1 / AT1G10220.1 / AT1G20360.1 / AT1G20320.1 / AT1G19630.1 / ... (1784 other terms not shown)	Searched on	Yes No Maybe Unverified Updated by ICAR Abstract Registration on 2008-09-11   Add Comment:
2.	gene AT3G53060.1 ( pub:12000) Alias: F8J2.230 Symbols: ASK6 <SKP1-like 6>  |  SK6 <SKP1-like 6> TAIR:gene:2085200 (Accession)	Article [ 45901 ] (2008) [abstract] Phenotypic analysis of ASK gene loss of function alleles in Arabidopsis   19th International Conference on Arabidopsis Research *Dezfulian, Mohammad H. Franczk, Marta Goettel, James Dahliwal, Rajdeep Crosby, William L. (TAIR Reference:501728110) Genetic studies have indicated the importance of targeted post-translational protein degradation as a key mechanism for the regulation of gene expression in plants. One of the main pathways involved in targeted protein degradation is the Ubiqutin/26S proteasome pathway. One highly studied class of E3 ligase are the SCF class whose quaternary structure typically includes 4 canonical polypeptides - Cullin, Rbx, Skp and an F-box subunit. In Arabidopsis, the Skp family of SCF subunits is encoded by 21 Skp1-like ASK genes compared to 1 orthologous gene in humans or yeast. Phylogenic analyses based on deduced amino acid sequence and expression profiles result in the clustering of the ASK gene family to distinct clades. The phylogeny suggests the possibility of functional redundancy between related Skp proteins. This suggestion is supported by genetic observation that ask1/ask2 double mutant exhibited a marked severe phenotype involving developmental delay and seedling lethality, whereas these defects were not detected in mutant lines carrying either ask1 or ask2 mutants alone(1). To elucidate the function of select members of the ASK gene family in Arabidopsis, we have undertaken a program of functional studies of a subset of the predicted ASK family of genes using two reverse-genetic resources; T-DNA insertions derived from the SIGNaL resource (2) targeting selected members of the gene family, and transgenic plants expressing artificial microRNA constructs designed to suppress the abundance of one or more ASK transcripts(3).Example data will be presented describing aberrant plant growth and development phenotypes among select loss-of-function alleles of ASK3 or ASK10 . The genetic resources developed by this study are the basis for further studies into the specific targeting functions and molecular mechanisms by which ASK genes contribute to plant patterning and development. 1.F. Liu et al., Plant Cell 16, 5 (2004). 2.J. M. Alonso et al., Science 301, 653 (2003). 3.R. Schwab, S. Ossowski, M. Riester, N. Warthmann, D. Weigel, Plant Cell 18, 1121 (2006). Not Scanned Other associated terms: AT2G25700.1 / AT2G03170.1 / AT3G53060.1 / AT3G21830.1 / AT3G21840.1 / AT3G21850.1 / AT3G21860.1 / AT3G25650.1 / AT4G34470.1 / AT5G42190.1 / AT1G20140.1 / AT1G75950.1 / growth / AT4G34210.1 / AT3G60010.1 / AT3G60020.1 / Arabidopsis / plant cell / gene expression / regulation of gene expression / AT1G17930 (MAIN) / AT2G25700 (SK3) / AT3G23130 (SUP) / AT3G21860 (SK10) / AT5G61670 (AtOR) / AT5G08590 (SNRK2.1) / AT5G42190 (SKP1B) / AT1G10940 (SNRK2.4) / AT1G75950 (SKP1) / ask1 / SUP / ask1 /	Searched on	Yes No Maybe Unverified Updated by ICAR Abstract Registration on 2008-09-11   Add Comment:
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